Journal: The Journal of comparative neurology

Article Title: Cell Death Atlas of the Postnatal Mouse Ventral Forebrain and Hypothalamus: Effects of Age and Sex

doi: 10.1002/cne.23298

Figure Lengend Snippet: Primary Antibodies

Article Snippet: The antibody concentration was based on the recommendations of the supplier as well as on data from a pilot study in which we ran a dilution curve. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antigen Immunogen Manufacturer data Dilution Activated caspase-3 Peptide sequence CRGTELDCGIETD, which is adjacent to Asp175 in human caspase-3 Cleaved caspase-3 antibody (polyclonal antibody raised in rabbit); Cell Signaling, 9661L, lot 36, Ref 08/2010 1:50,000 Calbindin D-28K Purified bovine kidney calbindin D-28K Anticalbindin D-28k antibody (monoclonal; derived from the CB-955 hybridoma produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine kidney calbinin D-28K); Sigma-Aldrich, C9848, lot 031M4859 1:10,000 Open in a separate window Primary Antibodies On the next day, tissue sections were rinsed in dilute blocking solution (1× TBS, 1% normal goat serum, and 0.02% Triton X) for 3 × 10 minutes, incubated for 1 hour in a secondary antibody solution (1× TBS, 2% normal goat serum, 0.32% Triton X, goat anti-rabbit biotinylated secondary antibody diluted 1:250 [Vector, Burlingame, CA; BA-1000]), washed in 1× TBS plus 0.2% Triton X solution for 3 × 10 minutes, and then incubated in ABC solution (Vectastain ABC-Elite Kit; Vector) for 1 hour.

Techniques: Sequencing, Purification, Derivative Assay, Produced